南瓜饲养榕树粉蚧的发育历期与生物学特征初报

榕树粉蚧是一种重要的入侵害虫,对果蔬和园林植物存在巨大的潜在威胁。为满足开展检疫处理、防控、生物学和生理学等研究的需要,本研究以南瓜为饲养材料,在室温条件下(26±1℃,RH 65%±5%)观察榕树粉蚧的生物学特性与发育历期。结果表明,榕树粉蚧具有典型的雌雄二型现象。雄虫主要经历卵、12龄若虫、预蛹、蛹、雄成虫阶段;雌虫经历卵、13龄若虫、雌成虫(产卵前期、产卵期)阶段,其发育历期(mean±SE)分别为8.51±0.03 d、9.79±0.09 d、7.46±0.10 d、6.52±0.11 d、12.13±0.11 d、8.16±0.11 d,世代发育历期54.4±0.25 d。雌成虫产卵量483651粒,平均550.87±51.07粒,卵期711 d,孵化率95.19%±0.75%。该研究结果表明榕树粉蚧繁殖能力强,应高度重视其防控工作。     

Combining ability of yield and yield components among Fusarium oxysporum f.sp. strigae-compatible and Striga-resistant sorghum genotypes

ABSTRACT

Use of sorghum [Sorghum bicolor (L.) Moench] cultivars with partial resistance to Striga spp. and Fusarium oxysporum f.sp. strigae (FOS) represents a novel strategy to control Striga. This study aimed to identify the nature of gene action controlling grain yield and yield components and to select promising sorghum crosses possessing both FOS compatibility and Striga resistance, along with good combining ability effects. One-hundred hybrids, developed from pairwise matings among 10 FOS compatible, high-yielding female lines and 10 Striga-resistant male lines, were evaluated with and without FOS inoculation. The F₁s were field evaluated at three locations in Tanzania known for their severe Striga infestation, using an alpha lattice design with two replications. General (GCA) and specific combining ability (SCA) variances were significant for grain yield per plant, hundred-seed weight, plant height, flowering time and the number of Striga plants. The study demonstrated FOS inoculation to be an effective means of controlling Striga. Families 675?×?672, AS435?×?3993 and 4643?×?AS436 displaying large SCA effects for grain yield, and 4567?×?AS429, 3424?×?AS430 and 3424?×?AS436 with small SCA effects for Striga counts should be useful genetic resources for breeding and integrated Striga management.     

玉米Reid种质DH系遗传分析

为探讨玉米Reid种质遗传改良效果,本研究以Reid类群重要种质J1491、J1492、J1493、J1495、J1498与PH6WC构建基础群体,通过DH技术育成DH系,经鉴定从中选取5个代表DH系6DH6、6DH7、6DH9、6DH10、6DH12作母本,以Non-Reid群5个优良自交系作父本,按NCII设计组配25个杂交组合,对DH系单株产量及其构成因素的杂种优势、配合力和相关方面进行了研究。结果表明,单株产量杂种优势以组合6DH7×J1628最高为180.22%,组合6DH12×J1673最低为54.94%;单株产量一般配合力效应值最高的有6DH7和6DH10,穗长、穗粗亦为正效应,因此认为6DH7和6DH10更具应用潜力;特殊配合力效应值最高的组合是6DH7×J1673。各产量性状中穗长、穗粗和秃尖长,狭义遗传率高,主要以基因加性效应为主。本研究为鉴定有潜在价值的DH系及杂交种提供选择,为玉米遗传改良提供参考。     

基于网络嵌入性的我国东北国有林区知识转移机理

文中从社会网络视角分析我国东北国有林区职工、家庭及林业企业知识转移的过程和影响因素，以期通过社会关系和网络结构促进林业企业转型。应用知识转移基本理论构建东北国有林区多层次知识转移理论模型，在此基础上从社会网络视角分析林区职工、家庭和林业企业3个层面的知识转移机理。研究表明:增强林区知识转移个体与利益相关者的联系程度、占据更多的结构洞位置以及提高个体网络中心度和凝聚程度有利于知识获取和内化；林区待岗职工与利益相关者的弱联系有利于其获得更多就业机会；国有森工企业由于过度嵌入社会网络，知识内化和创新动力不足。     

A data transfer method for improving seed identification of maize (Zea mays) haploid breeding based on genetic similarity

Maize haploid breeding technology is able to identify haploid seeds non‐destructively, rapidly and at low cost with the help of Near‐infrared (NIR) spectral analysis. However, due to the hybridization of numerous parents and the low production rate of haploid, the haploid data collection becomes a burden for engineering this technology. Biologically, there are considerable similarities between the progeny of the same female parent and different male parents. Based on this advantage, similar spectral data can be transferred when the NIR technology is employed. A revised method of Transfer adaptive boost (TrAdaBoost) is proposed to improve identifying for the backpropagation neural network (BPNN) classifier. To avoid the negative transfer, a screening thresh is used to select out similar data, and the amount of these data are limited to join current training. The results show that the identification performances are improved significantly when the data amount is small. This method shows a high ability to make the seed identification more convenient for engineering maize haploid breeding.     

土地规模化经营能否促进农业社会化服务获取?——基于全国3类农户样本的实证分析

土地流转和农业社会化服务是经营规模化和服务规模化的根本途径,研究二者关系对农业供给侧结构性改革、农业现代化发展乃至乡村振兴具有重要意义。基于第二期"全国新型农业经营主体发展指数调查"数据,运用负二项回归模型实证分析土地流转及转入不同特征土地对普通农户、专业大户和家庭农场农业社会化服务获取的影响。结果表明,单纯的土地转入对不同类型农户农业社会化服务获取均不具有显著影响。但如果转入土地有利于规模化经营、农业基础设施较好,则能显著促进社会化服务获取。另外,农户经营能力越强,对不同类型社会化服务的应用程度也越高。因此,政府应推动更有效率的土地流转,实现农业适度规模经营;加强农业社会化服务供给,提升农户生产经营效率;强化经营主体培训,增强联结带动能力。     

轻型木结构墙体稳态传热性能及其保温材料影响比较

轻型木结构墙体的热工性能对建筑节能至关重要,其稳态传热性能是节能效率评估指标之一。工程调研发现,轻型木结构墙体墙骨柱热传递过程易产生冷热桥,改善墙体保温构造并通过试验测试,对得到不同构造的墙体传热系数等指标进行分析研究,有利于设计人员优选墙体设计参数、研发墙体新型保温材料。试验采用标定热箱法与热流计法相结合的试验方法——控温箱-热流计法对改善保温措施的墙体进行试验,分析了12种墙体试件的热流密度、传热系数、热阻以及日传热量参数,并与理论计算结果进行比较验证,试验结果与理论计算结果吻合性较好。研究发现:气凝胶毡放置在墙骨柱位置能够有效降低平均传热系数3.8%~15.0%,随厚度增加可逐渐减弱或消除墙骨柱冷热桥效应;聚苯乙烯保温板可有效降低传热系数32.4%~35.1%,墙体蓄热能力较好;纳米纤维保温材料可有效降低传热系数5.9%~8.7%,提高墙体冷热面温差,平衡室内温度。选取的3种保温材料中,聚苯乙烯保温板相比气凝胶毡和纳米纤维保温材料,对墙体整体热工性能的提升更为显著。     

Bamboo NiR gene is associated with regeneration capacity

It was reported that the activity of NiR (nitrite reductase) enzyme encoded by the NiR gene was correlated with the regeneration ability in rice. To testify the function of NiR gene in bamboo, seven bamboo species whose calli had different differentiation rate were chosen to analyse their NiR enzyme activity. The results showed that bamboo NiR enzymatic activity had a certain correlation with the regeneration capacity. A NiR gene named DhNiR from Dendrocalamus hamiltonii with high regeneration capacity was cloned. Sequence analysis revealed that the ORF of DhNiR was 1779bp encoding 592 amino acids. Overexpression of DhNiR in rice reduced the time of shoot differentiation and increased the transformation rate, suggesting that DhNiR might play an important role in the regeneration ability of bamboo, and can be applied in regeneration and gene transformation of bamboo and other plant species.     

一株耐铝甜瓜枯萎病拮抗细菌的分离鉴定及其耐铝特性研究

从甜瓜根际酸性土壤中分离筛选到1株耐铝甜瓜枯萎病拮抗菌，命名为A2。根据表型、生理生化特征和16S rRNA基因序列相似性分析，将其鉴定为Pseudomonas sp.。菌株A2对甜瓜枯萎病病原菌的相对防效为68.3%，且拮抗能力具有遗传稳定性。相比AlCl3处理，菌株A2对Al2(SO4)3表现出了更好的耐受性，最高可耐受50mmol/L Al3+。在含有A13+的S-LB培养基中，菌株最适生长温度为30 ℃；培养基初始pH值的降低会加剧A13+对菌株A2的毒害作用。研究结果可为含活性铝的酸性土壤中甜瓜枯萎病的生物防治提供优良菌株资源和理论基础。     

杂草环境下转EPSPS基因大豆NZL06-698的生态适应性研究

为研究转EPSPS基因大豆NZL06-698在杂草环境中的生态适应性,试验设置杂草处理模拟野外环境,研究转基因大豆NZL06-698(GT698)的生存竞争能力以及外源EPSPS蛋白表达情况。结果表明:在相同处理下转基因大豆GT698的株高(R8期)、百粒重显著高于亲本大豆蒙豆12(MD12),但单株产量、单株籽粒数、结实率显著低于亲本大豆,说明外源基因的存在并未增强转基因大豆的生存竞争力,且转基因大豆在野外的生存竞争能力与亲本大豆相比较弱。试验注意到,杂草环境对转EPSPS基因大豆的生长有明显的限制作用,杂草处理下转基因大豆的单株产量、单株籽粒数、百粒重、结实率等指标均显著低于对照处理。ELISA检测结果表明,转基因大豆叶片及籽粒中外源EPSPS蛋白在杂草处理及对照中均正常表达,且两种处理下的EPSPS蛋白表达量无显著差异。以上结果表明杂草环境中转基因大豆的EPSPS蛋白正常表达,正常提供抗草甘膦性状,但是外源基因的存在并没有增加转基因大豆的生存竞争能力,且转基因大豆的生长受到杂草环境的显著抑制,由此得出结论:与亲本大豆相比,转EPSPS基因大豆NZL06-698在野外环境中生态适应能力较弱。